Combat of iron-deprivation through a plant growth promoting fluorescent Pseudomonas strain GRP3A in mung bean (Vigna radiata L. Wilzeck)

Microbiol Res. 2003;158(1):77-81. doi: 10.1078/0944-5013-00182.

Abstract

Microorganisms and plants sustain themselves under iron-deprived conditions by releasing siderophores. Among others, fluorescent pseudomonads are known to exert extensive biocontrol action against soil and root borne phytopathogens through release of antimicrobials and siderophores. In this study, production and regulation of siderophores by fluorescent Pseudomonas strain GRP3A was studied. Among various media tested, standard succinate medium (SSM) promoted maximum siderophore production of 56.59 mg l(-1). There were low levels of siderophore in complex media like King's B medium, trypticase soya medium and nutrient medium (41.27, 29.86 and 27.63 mg l(-1)), respectively. In defferrated SSM, siderophore level was quantified to be 68.74 mg l(-1). Supplementation with iron (FeCl3) resulted in decreased siderophore levels depending on concentration. Siderophore production was promoted by Zn2+ (78.94 mg l(-1)), Cu2+ (68.80 mg l(-1)) whereas Co2+ (57.33 mg l(-1)) and Fe3+ reduced siderophore production (37.44 mg l(-1) as compared to control (55.97 mg l(-1)). Strain GRP3A showed plant growth promotion under iron limited conditions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbon / pharmacology
  • Chlorides
  • Culture Media / chemistry
  • Culture Media / pharmacology
  • Fabaceae / drug effects
  • Fabaceae / growth & development
  • Fabaceae / microbiology*
  • Ferric Compounds / pharmacology
  • Fluorescence
  • Iron / deficiency
  • Iron / metabolism
  • Iron / pharmacology*
  • Pseudomonas fluorescens / drug effects
  • Pseudomonas fluorescens / growth & development*
  • Pseudomonas fluorescens / metabolism
  • Siderophores / biosynthesis
  • Succinic Acid / pharmacology
  • Trace Elements / pharmacology

Substances

  • Chlorides
  • Culture Media
  • Ferric Compounds
  • Siderophores
  • Trace Elements
  • Carbon
  • Succinic Acid
  • Iron
  • ferric chloride