Expression profiling of the host response to bacterial infection: the transition from basal to induced defence responses in RPM1-mediated resistance

Plant J. 2003 Feb;33(4):665-76. doi: 10.1046/j.1365-313x.2003.01653.x.


Changes in transcription in leaves of Arabidopsis thaliana were characterised following challenge with strains of Pseudomonas syringae pv. tomato DC3000 allowing differentiation of basal resistance (hrpA mutants), gene-specific resistance (RPM1-specified interactions) and susceptibility (wild-type pathogen). In planta avirulence gene induction, changes in host [Ca2+]cyt and leaf collapse were used to delineate the transition from infection to induced resistance. The plant responds rapidly, dynamically and discriminately to infection by phytopathogenic bacteria. Within the first 2 h host transcriptional changes are common to all challenges indicating that Type III effector function does not contribute to early events in host transcriptome re-programming. The timing of induction for specific transcripts was reproducible, hierarchical and modulated at least in part through EDS1 function. R gene-specific transcripts were not observed until 3 h after inoculation. Intriguingly, the R gene-specific response proteins are expected to localise to diverse cellular addresses indicative of a global impact on cellular homeostasis. The altered transcriptional response rapidly manifests into initial symptoms of leaf collapse within 2 h, although establishment of the full macroscopic HR occurs significantly later.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / genetics*
  • Arabidopsis / microbiology
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism
  • Bacteria / growth & development*
  • Bacteria / pathogenicity
  • Calcium / metabolism
  • DNA, Complementary / genetics
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Gene Expression Profiling*
  • Gene Expression Regulation, Plant
  • Immunity, Innate / genetics*
  • Immunity, Innate / physiology
  • Plant Diseases / genetics*
  • Plant Diseases / microbiology
  • Polymorphism, Restriction Fragment Length
  • RNA, Plant / genetics
  • RNA, Plant / metabolism
  • Signal Transduction / genetics
  • Signal Transduction / physiology
  • Transcriptional Activation


  • Arabidopsis Proteins
  • DNA, Complementary
  • DNA-Binding Proteins
  • EDS1 protein, Arabidopsis
  • RNA, Plant
  • RPM1 protein, Arabidopsis
  • Calcium