Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell protein localizations

J Cell Biol. 2003 Mar 3;160(5):629-33. doi: 10.1083/jcb.200210140.


The current advances in fluorescence microscopy, coupled with the development of new fluorescent probes, make fluorescence resonance energy transfer (FRET) a powerful technique for studying molecular interactions inside living cells with improved spatial (angstrom) and temporal (nanosecond) resolution, distance range, and sensitivity and a broader range of biological applications.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Eukaryotic Cells / cytology*
  • Eukaryotic Cells / metabolism
  • Fluorescence Resonance Energy Transfer / methods*
  • Fluorescence Resonance Energy Transfer / trends
  • Fluorescent Dyes*
  • Green Fluorescent Proteins
  • Humans
  • Image Processing, Computer-Assisted / methods
  • Image Processing, Computer-Assisted / trends
  • Luminescent Proteins
  • Microscopy, Fluorescence / methods*
  • Microscopy, Fluorescence / trends
  • Proteins / metabolism*


  • Fluorescent Dyes
  • Luminescent Proteins
  • Proteins
  • Green Fluorescent Proteins