Determination of free phenolic acids in wort and beer by coulometric array detection

J Agric Food Chem. 2003 Mar 12;51(6):1548-54. doi: 10.1021/jf0260040.


The simultaneous determination of 19 phenolic compounds was performed directly in wort and beer by a combination of reverse-phase high-performance liquid chromatography coupled with coulometric array detection. Chromatographic separation was achieved with an appropriate gradient of flow and a binary solvent based on phosphate buffer, methanol, and acetonitrile in a 45-min run. Eight serial coulometric detectors were used for on-line generation of voltammetric data to resolve coeluting compounds. The method was reliable and sensitive, the regression coefficient of standard calibration curves is 0.972 < or = r < or = 1.000, and the standard deviation value ranges from 0.010 to 0.129 mg/L for wort and from 0.002 to 0.332 mg/L for beer. The mean concentrations of phenolic acids were 22.1 and 33.8 mg/L, respectively, in worts and beers produced in Italy. These amounts represent 5 and 10% of the non-tannic, non-flavonoid phenols in wort and beer, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Beer / analysis*
  • Chromatography, High Pressure Liquid / methods*
  • Hydroxybenzoates / analysis*
  • Italy
  • Phenols / analysis
  • Sensitivity and Specificity


  • Hydroxybenzoates
  • Phenols
  • phenolic acid