Ontogeny of semaphorins 3A and 3F and their receptors neuropilins 1 and 2 in the kidney

Gene Expr Patterns. 2002 Nov;2(1-2):151-5. doi: 10.1016/s0925-4773(02)00305-2.

Abstract

Semaphorins 3A and 3F are axon guidance proteins during nervous system development. Their expression pattern and function outside the nervous system are unknown. Neuropilin 1 and 2 (NP-1, NP-2) are natural ligands for semaphorins 3A and 3F, respectively. NP-1 is also a co-receptor for vascular endothelial growth factor (VEGF) required for normal vascular development. We showed that VEGF is a direct chemoattractant for glomerular endothelial cells towards developing nephrons. To examine whether semaphorins could modulate VEGF endothelial cell guidance cues in the developing kidney, we studied the expression of semaphorin 3A and semaphorin 3F and their receptors NP-1 and NP-2 in the kidney during ontogeny using Northern blot analysis, in situ hybridization, Western blot analysis and immunohistochemistry. All four genes are developmentally regulated, with abundant expression during organogenesis and downregulation in the adult kidney. Semaphorin 3A and 3F are expressed by podocytes and tubules whereas their receptors NP-1 and NP-2 are localized to endothelial cells. In vitro, renal tubular epithelial cell lines (tsMPT, IRPT and MDCK) and glomerular endothelial cells express both semaphorins and their receptors, suggesting the presence of an autocrine system. The distribution of the receptors NP-1 and NP-2 in endothelial cells and developing vessels is complementary to that of the ligands in adjacent epithelial cells during kidney development. The sum of the guidance cues provided by VEGF and semaphorins 3A and 3F may be important determinants of the pattern of endothelial cell migration during kidney morphogenesis.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Blotting, Northern
  • Gene Expression Profiling
  • Kidney / embryology
  • Kidney / metabolism*
  • Membrane Proteins / biosynthesis*
  • Membrane Proteins / metabolism
  • Mice
  • Nerve Tissue Proteins / biosynthesis*
  • Nerve Tissue Proteins / metabolism
  • Neuropilin-1 / biosynthesis*
  • Neuropilin-1 / metabolism
  • Neuropilin-2 / biosynthesis*
  • Neuropilin-2 / metabolism
  • Rats
  • Semaphorin-3A / biosynthesis*
  • Semaphorin-3A / metabolism

Substances

  • Membrane Proteins
  • Nerve Tissue Proteins
  • Neuropilin-2
  • Sema3f protein, mouse
  • Semaphorin-3A
  • Neuropilin-1