Carbonic Anhydrase Inhibitors: SAR and X-ray Crystallographic Study for the Interaction of Sugar sulfamates/sulfamides With Isozymes I, II and IV

Bioorg Med Chem Lett. 2003 Mar 10;13(5):841-5. doi: 10.1016/s0960-894x(03)00029-5.

Abstract

A series of sugar sulfamate/sulfamide derivatives were prepared and assayed as inhibitors of three carbonic anhydrase (CA) isozymes, hCA I, hCA II and bCA IV. Best inhibitory properties were observed for the clinically used antiepileptic drug topiramate, which is a low nanomolar CA II inhibitor, and possesses good inhibitory properties against the other two isozymes investigated here, similarly with acetazolamide, methazolamide or dichlorophenamide. The X-ray structure of the complex of topiramate with hCA II has been solved and it revealed a very tight association of the inhibitor, with a network of seven strong hydrogen bonds fixing topiramate within the active site, in addition to the Zn(II) coordination through the ionized sulfamate moiety. Structural changes in this series of sugar derivatives led to compounds with diminished CA inhibitory properties as compared to topiramate.

MeSH terms

  • Animals
  • Binding Sites
  • Carbonic Anhydrase I / antagonists & inhibitors
  • Carbonic Anhydrase II / antagonists & inhibitors
  • Carbonic Anhydrase IV / antagonists & inhibitors
  • Carbonic Anhydrase Inhibitors / chemistry*
  • Carbonic Anhydrase Inhibitors / metabolism
  • Carbonic Anhydrase Inhibitors / pharmacology*
  • Cattle
  • Crystallography, X-Ray
  • Fructose / analogs & derivatives*
  • Fructose / chemistry
  • Fructose / metabolism
  • Humans
  • Hydrogen Bonding
  • Models, Molecular
  • Structure-Activity Relationship
  • Sulfonamides / chemistry*
  • Sulfonamides / metabolism
  • Sulfonamides / pharmacology*
  • Sulfonic Acids / chemistry*
  • Sulfonic Acids / metabolism
  • Sulfonic Acids / pharmacology*
  • Topiramate
  • Zinc / chemistry
  • Zinc / metabolism

Substances

  • Carbonic Anhydrase Inhibitors
  • Sulfonamides
  • Sulfonic Acids
  • Topiramate
  • Fructose
  • sulfamic acid
  • Carbonic Anhydrase I
  • Carbonic Anhydrase II
  • Carbonic Anhydrase IV
  • Zinc