MicroSAGE is highly representative and reproducible but reveals major differences in gene expression among samples obtained from similar tissues

Genome Biol. 2003;4(3):R17. doi: 10.1186/gb-2003-4-3-r17. Epub 2003 Feb 18.


Background: Serial analysis of gene expression using small amounts of starting material (microSAGE) has not yet been conclusively shown to be representative, reproducible or accurate.

Results: We show that microSAGE is highly representative, reproducible and accurate, but that pronounced differences in gene expression are seen between tissue samples taken from different individuals.

Conclusions: MicroSAGE is a reliable method of comprehensively profiling differences in gene expression among samples, but care should be taken in generalizing results obtained from libraries constructed from tissue obtained from different individuals and/or processed or stored differently.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.
  • Validation Study

MeSH terms

  • 3T3 Cells
  • Adult
  • Age Factors
  • Aged
  • Aged, 80 and over
  • Animals
  • Cell Line
  • Computers, Molecular
  • Female
  • Gene Expression Profiling / methods*
  • Gene Library
  • Genes, cdc
  • Genetic Variation / genetics*
  • Humans
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Oligonucleotide Array Sequence Analysis / methods
  • Organ Specificity / genetics
  • Retina / chemistry
  • Retina / metabolism
  • Sex Factors