Transposition of the Drosophila hydei Minos transposon in the mouse germ line

Genomics. 2003 Feb;81(2):108-11. doi: 10.1016/s0888-7543(02)00030-7.


We tested the suitability of the fly transposon Minos, a member of the Tc1/mariner superfamily, for insertional mutagenesis in the mouse germ line. We generated a transgenic mouse line expressing Minos transposase in growing oocytes and another carrying a tandem array of nonautonomous transposons. The frequency of transposition in the progeny derived from oocytes carrying both transgenes is 8.2%. Analysis of the new integration sites shows a high frequency of transpositions to a different chromosome. Thus Minos transposition could be an effective system for insertional mutagenesis and functional genomic analysis in the mouse.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Southern
  • DNA Transposable Elements*
  • Drosophila / genetics*
  • In Situ Hybridization, Fluorescence
  • Mice


  • DNA Transposable Elements