Improved anaerobic use of arginine by Saccharomyces cerevisiae

Appl Environ Microbiol. 2003 Mar;69(3):1623-8. doi: 10.1128/AEM.69.3.1623-1628.2003.


Anaerobic arginine catabolism in Saccharomyces cerevisiae was genetically modified to allow assimilation of all four rather than just three of the nitrogen atoms in arginine. This was accomplished by bypassing normal formation of proline, an unusable nitrogen source in the absence of oxygen, and causing formation of glutamate instead. A pro3 ure2 strain expressing a PGK1 promoter-driven PUT2 allele encoding Delta(1)-pyrroline-5-carboxylate dehydrogenase lacking a mitochondrial targeting sequence produced significant cytoplasmic activity, accumulated twice as much intracellular glutamate, and produced twice as much cell mass as the parent when grown anaerobically on limiting arginine as sole nitrogen source.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Anaerobiosis
  • Arginine / metabolism*
  • Culture Media
  • Gene Expression Regulation, Fungal
  • Genetic Engineering / methods*
  • Glutamic Acid / metabolism
  • Glutathione Peroxidase
  • Nitrogen / metabolism
  • Prions / genetics
  • Prions / metabolism
  • Pyrroline Carboxylate Reductases / genetics
  • Pyrroline Carboxylate Reductases / metabolism
  • Saccharomyces cerevisiae / growth & development*
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism


  • Culture Media
  • Prions
  • Saccharomyces cerevisiae Proteins
  • Glutamic Acid
  • Arginine
  • Glutathione Peroxidase
  • URE2 protein, S cerevisiae
  • Pyrroline Carboxylate Reductases
  • delta-1-pyrroline-5-carboxylate reductase
  • Nitrogen