Abstract
This report compares the performances of two popular genotypic methods used for tracking the sources of fecal pollution in water, ribotyping and repetitive extragenic palindromic-PCR (rep-PCR). The rep-PCR was more accurate, reproducible, and efficient in associating DNA fingerprints of fecal Escherichia coli with human and animal hosts of origin.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Cattle
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DNA Fingerprinting / methods
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DNA, Bacterial / analysis
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Dogs
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Escherichia coli / classification*
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Escherichia coli / genetics
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Feces / microbiology
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Humans
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Polymerase Chain Reaction / methods*
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Reproducibility of Results
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Ribotyping*