Antigenic hepatitis A virus structures may be produced in Escherichia coli

Glòria Sánchez; Santiago Caballero; Susana Guix; Albert Bosch; Rosa M Pintó

doi:10.1128/AEM.69.3.1840-1843.2003

Antigenic hepatitis A virus structures may be produced in Escherichia coli

Appl Environ Microbiol. 2003 Mar;69(3):1840-3. doi: 10.1128/AEM.69.3.1840-1843.2003.

Authors

Glòria Sánchez¹, Santiago Caballero, Susana Guix, Albert Bosch, Rosa M Pintó

Affiliation

¹ Enteric Virus Laboratory, Department of Microbiology, University of Barcelona, Av. Diagonal 645, 08028 Barcelona, Spain.

Abstract

The synthesis of 14S pentamers and 70S empty capsids of hepatitis A virus (HAV) has been accomplished by expressing the viral genome for periods of time longer than 4 h in Escherichia coli. HAV pentamers (14S) self-assembled into capsids (70S) in vitro. The antibodies induced by these structures recognized and neutralized HAV.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Capsid / chemistry
Capsid / immunology
Capsid / metabolism
Escherichia coli / genetics*
Female
Hepatitis A Antibodies / blood
Hepatitis A Antigens / immunology
Hepatitis A Antigens / metabolism*
Hepatitis A virus / immunology
Hepatitis A virus / metabolism
Mice
Neutralization Tests
Plasmids

Substances

Hepatitis A Antibodies
Hepatitis A Antigens