Comparison of methods for DNA isolation from food samples for detection of Shiga toxin-producing Escherichia coli by real-time PCR

Appl Environ Microbiol. 2003 Mar;69(3):1844-6. doi: 10.1128/AEM.69.3.1844-1846.2003.


In this study, food samples were intentionally contaminated with Escherichia coli O157:H7, and then DNA was isolated by using four commercial kits. The isolated DNA samples were compared by using real-time PCR detection of the Shiga toxin genes. The four kits tested worked similarly.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Bread / microbiology
  • Cattle
  • DNA, Bacterial / analysis
  • DNA, Bacterial / isolation & purification*
  • Escherichia coli O157 / genetics
  • Escherichia coli O157 / isolation & purification*
  • Escherichia coli O157 / metabolism
  • Food Contamination
  • Meat Products / microbiology
  • Reagent Kits, Diagnostic*
  • Shiga Toxin / biosynthesis
  • Shiga Toxin / genetics*
  • Vegetables / microbiology


  • DNA, Bacterial
  • Reagent Kits, Diagnostic
  • Shiga Toxin