Comparison of methods for DNA isolation from food samples for detection of Shiga toxin-producing Escherichia coli by real-time PCR

Loree C Heller; Carisa R Davis; K Kealy Peak; David Wingfield; Andrew C Cannons; Philip T Amuso; Jacqueline Cattani

doi:10.1128/AEM.69.3.1844-1846.2003

Comparison of methods for DNA isolation from food samples for detection of Shiga toxin-producing Escherichia coli by real-time PCR

Appl Environ Microbiol. 2003 Mar;69(3):1844-6. doi: 10.1128/AEM.69.3.1844-1846.2003.

Authors

Loree C Heller¹, Carisa R Davis, K Kealy Peak, David Wingfield, Andrew C Cannons, Philip T Amuso, Jacqueline Cattani

Affiliation

¹ Esoteric Testing, Department of Pathology, Tampa General Hospital, PO Box 1289, Tampa, FL 33101, USA. lheller@tgh.org

Abstract

In this study, food samples were intentionally contaminated with Escherichia coli O157:H7, and then DNA was isolated by using four commercial kits. The isolated DNA samples were compared by using real-time PCR detection of the Shiga toxin genes. The four kits tested worked similarly.

Publication types

Comparative Study
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Bread / microbiology
Cattle
DNA, Bacterial / analysis
DNA, Bacterial / isolation & purification*
Escherichia coli O157 / genetics
Escherichia coli O157 / isolation & purification*
Escherichia coli O157 / metabolism
Food Contamination
Meat Products / microbiology
Reagent Kits, Diagnostic*
Shiga Toxin / biosynthesis
Shiga Toxin / genetics*
Vegetables / microbiology

Substances

DNA, Bacterial
Reagent Kits, Diagnostic
Shiga Toxin