Cr(VI) reduction by sulfidogenic and nonsulfidogenic microbial consortia

Appl Environ Microbiol. 2003 Mar;69(3):1847-53. doi: 10.1128/AEM.69.3.1847-1853.2003.

Abstract

In time course experiments, bacterial community compositions were compared between a sulfidogenic and two nonsulfidogenic Cr(VI)-reducing consortia enriched from metal-contaminated sediments. The consortia were subjected to 0 and 0.85 mM or 1.35 mM Cr(VI), and Cr(VI) reduction, growth, and denaturing gradient gel electrophoresis profiles of PCR products of small-subunit (16S) ribosomal genes were compared. Results showed that although Cr(VI) was completely reduced by the three consortia, Cr(VI) inhibited cell growth, with sulfate-reducing bacteria being particularly sensitive to Cr(VI) toxicity relative to other bacteria in the consortia.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacteria, Anaerobic / classification
  • Bacteria, Anaerobic / genetics
  • Bacteria, Anaerobic / metabolism*
  • Chromium / metabolism*
  • Culture Media
  • Ecosystem*
  • Electrophoresis / methods
  • Geologic Sediments / microbiology
  • Oxidation-Reduction
  • Phylogeny
  • Polymerase Chain Reaction
  • Seawater
  • Sequence Analysis, DNA
  • Soil Microbiology
  • Sulfur-Reducing Bacteria / classification
  • Sulfur-Reducing Bacteria / genetics
  • Sulfur-Reducing Bacteria / metabolism*
  • Water Pollution, Chemical

Substances

  • Culture Media
  • Chromium
  • chromium hexavalent ion