Large-scale isolation of CD133+ progenitor cells from G-CSF mobilized peripheral blood stem cells

Bone Marrow Transplant. 2003 Jan;31(1):17-22. doi: 10.1038/sj.bmt.1703792.


We have evaluated the feasibility of large-scale isolation of CD133+ progenitors from healthy mobilized adult donors for potential clinical use in autologous and allogeneic transplantation. A total of 11 healthy volunteer adult donors were mobilized with G-CSF. CD133+ stem cells were isolated from a single leukapheresis using the Clinimacs method. The median percentage of CD133 before positive selection was 0.75% (range 0.39-2.03%). After selection, the median purity and recovery was 94% (range 85.2-98.0%) and 69% (range 44-100%), respectively. The median log10 T-cell depletion obtained by CD133+ positive selection was 4.2 (range 3.8-4.7). The CD133+ progenitors were highly enriched in colony-forming units (CFU) and transplantation into NOD/SCID mice resulted in a high engraftment rate. Transplantation of sorted CD133+/CD34+ cells into NOD/SCID mice showed a higher engraftment compared to CD133-/CD34+ cells. Mobilized peripheral CD133+ stem cells can be purified in large scale for potential clinical use. The biological function of the cells is not impaired. The majority of the NOD/SCID repopulating cells are within the CD133+/CD34+ subpopulation. Therefore, clinical studies using purified CD133+ stem cells can be envisoned to further clarify the role of CD133+ stem cells in hematopoietic reconstitution after transplantation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • AC133 Antigen
  • Adult
  • Animals
  • Antigens, CD / blood
  • Cell Separation
  • Colony-Forming Units Assay
  • DNA Primers
  • Filgrastim
  • Flow Cytometry
  • Glycoproteins / blood
  • Granulocyte Colony-Stimulating Factor / therapeutic use*
  • Hematopoietic Stem Cell Mobilization / methods*
  • Hematopoietic Stem Cells / cytology
  • Hematopoietic Stem Cells / immunology
  • Humans
  • Leukapheresis
  • Living Donors
  • Mice
  • Mice, Inbred NOD
  • Mice, SCID
  • Peptides / blood
  • Polymerase Chain Reaction / methods
  • Recombinant Proteins
  • Transplantation, Heterologous


  • AC133 Antigen
  • Antigens, CD
  • DNA Primers
  • Glycoproteins
  • PROM1 protein, human
  • Peptides
  • Prom1 protein, mouse
  • Recombinant Proteins
  • Granulocyte Colony-Stimulating Factor
  • Filgrastim