Modulation of oxidative stress in response to gamma-radiation in human glioma cell lines

J Neurooncol. 2003 Jan;61(2):89-94. doi: 10.1023/a:1022168805198.


Radiation therapy is routinely used in the management of primary central nervous system malignancies. However, the efficacy of this therapeutic modality is limited by the occurrence of resistance. In the present study, we investigated whether modulation of oxidative stress might underlie glioma cell radioresistance. Superoxide dismutase activity in irradiated M059J cells was two-fold higher than that in untreated controls, but did not significantly change in U-87 and U-138 cells. This is accompanied by an increase in reactive oxygen species content and decreases in cells viability. Pharmacological or genetic modulation of oxidative stress could be associated with an enhancement in the susceptibility of tumor cells to radiation therapy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain Neoplasms / enzymology*
  • Catalase / metabolism
  • Cell Survival
  • Free Radicals / metabolism
  • Gamma Rays
  • Gene Expression
  • Glioma / enzymology*
  • Oxidative Stress*
  • Radiation Tolerance
  • Rats
  • Reactive Oxygen Species / metabolism*
  • Superoxide Dismutase / metabolism*
  • Thiobarbituric Acid Reactive Substances / metabolism
  • Transfection
  • Tumor Cells, Cultured / radiation effects


  • Free Radicals
  • Reactive Oxygen Species
  • Thiobarbituric Acid Reactive Substances
  • Catalase
  • Superoxide Dismutase