Abstract
Using sodium cyanide (NaCN) and glucose deprivation induced cell injury in PC12 as an injury model, we investigated the protective effects of pulsatilloside A and anemoside A 3 on neurons. The results showed that PC12 cells under the NaCN-injury and glucose deprivation would undergo apoptosis. Additions of pulsatilloside A and anemoside A 3, at dosages ranging from 0.1, 1 and 10 microg/ml, protected PC12 cells from apoptosis determined by MTT, LDH release analysis, and flow cytometry measurement.
Publication types
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Letter
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Research Support, Non-U.S. Gov't
MeSH terms
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Alzheimer Disease / drug therapy
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Animals
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Apoptosis / drug effects*
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Dose-Response Relationship, Drug
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Flow Cytometry
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Glucose
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Humans
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Neuroprotective Agents / administration & dosage
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Neuroprotective Agents / pharmacology*
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Neuroprotective Agents / therapeutic use
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PC12 Cells / drug effects
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Phytotherapy*
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Plant Extracts / administration & dosage
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Plant Extracts / isolation & purification
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Plant Extracts / pharmacology*
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Plant Extracts / therapeutic use
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Plant Roots
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Pulsatilla*
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Rats
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Saponins
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Sodium Cyanide
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Triterpenes
Substances
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Neuroprotective Agents
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Plant Extracts
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Pulsatilloside A
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Saponins
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Triterpenes
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anemoside A3
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Glucose
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Sodium Cyanide