Pulsatilloside A and anemoside A3 protect PC12 cells from apoptosis induced by sodium cyanide and glucose deprivation

Planta Med. 2003 Feb;69(2):171-4. doi: 10.1055/s-2003-37705.

Abstract

Using sodium cyanide (NaCN) and glucose deprivation induced cell injury in PC12 as an injury model, we investigated the protective effects of pulsatilloside A and anemoside A 3 on neurons. The results showed that PC12 cells under the NaCN-injury and glucose deprivation would undergo apoptosis. Additions of pulsatilloside A and anemoside A 3, at dosages ranging from 0.1, 1 and 10 microg/ml, protected PC12 cells from apoptosis determined by MTT, LDH release analysis, and flow cytometry measurement.

Publication types

  • Letter
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alzheimer Disease / drug therapy
  • Animals
  • Apoptosis / drug effects*
  • Dose-Response Relationship, Drug
  • Flow Cytometry
  • Glucose
  • Humans
  • Neuroprotective Agents / administration & dosage
  • Neuroprotective Agents / pharmacology*
  • Neuroprotective Agents / therapeutic use
  • PC12 Cells / drug effects
  • Phytotherapy*
  • Plant Extracts / administration & dosage
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology*
  • Plant Extracts / therapeutic use
  • Plant Roots
  • Pulsatilla*
  • Rats
  • Saponins
  • Sodium Cyanide
  • Triterpenes

Substances

  • Neuroprotective Agents
  • Plant Extracts
  • Pulsatilloside A
  • Saponins
  • Triterpenes
  • anemoside A3
  • Glucose
  • Sodium Cyanide