Genome imprinting regulated by the mouse Polycomb group protein Eed

Nat Genet. 2003 Apr;33(4):502-7. doi: 10.1038/ng1125. Epub 2003 Mar 10.

Abstract

Epigenetic regulation is essential for temporal, tissue-specific and parent-of-origin-dependent gene expression. It has recently been found that the mouse Polycomb group (PcG) gene Eed (embryonic ectoderm development) acts to maintain repression of the imprinted X chromosome. Here, we investigated whether Eed is also required for regulation of autosomal imprinted loci. Expression analyses showed that transcripts from the silent alleles of a subset of paternally repressed genes were present in Eed(-/-) embryos. Parent-of-origin methylation was preserved in these embryos, but we observed changes in the methylation status of specific CpGs in differentially methylated regions (DMRs) at affected but not at unaffected loci. These data identify Eed as a member of a new class of trans-acting factors that regulate parent-of-origin expression at imprinted loci.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • Animals
  • Base Sequence
  • CpG Islands
  • DNA Methylation
  • Genomic Imprinting*
  • Heterozygote
  • Mice
  • Models, Genetic
  • Molecular Sequence Data
  • Polycomb Repressive Complex 2
  • Repressor Proteins / genetics*
  • Repressor Proteins / physiology*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sulfites / pharmacology
  • Transcription, Genetic
  • X Chromosome

Substances

  • Eed protein, mouse
  • Repressor Proteins
  • Sulfites
  • Polycomb Repressive Complex 2

Associated data

  • GENBANK/AB003040
  • GENBANK/AB047760
  • GENBANK/AF119385
  • GENBANK/AF147785
  • GENBANK/AF309654
  • GENBANK/AF332579
  • GENBANK/AK003004
  • GENBANK/AL663087
  • GENBANK/AP001293
  • GENBANK/BC019520
  • GENBANK/U70068
  • GENBANK/Y13832
  • RefSeq/NM_009434
  • RefSeq/NM_010514
  • RefSeq/NM_013123