[Diagnosis of imported malaria with multiplex PCR on LighCycler apparatus]

Pathol Biol (Paris). 2003 Feb;51(1):44-6. doi: 10.1016/s0369-8114(02)00319-x.
[Article in French]

Abstract

For the diagnosis of imported malaria, a competitive multiplex PCR using LightCycler was developed and compared for 3 months with a traditional PCR method. Hundred eighty three patients were tested by these 2 techniques of molecular diagnosis: 60 were positive for P. falciparum, 9 were positive for other Plasmodium species and 114 were negative. The LightCycler method was found in total agreement with the traditional PCR.

Publication types

  • Comparative Study
  • English Abstract

MeSH terms

  • Animals
  • DNA, Protozoan / analysis
  • Humans
  • Malaria / diagnosis*
  • Plasmodium / genetics
  • Plasmodium falciparum / genetics
  • Polymerase Chain Reaction / instrumentation*

Substances

  • DNA, Protozoan