Highly pyrimethamine-resistant alleles of dihydrofolate reductase in isolates of Plasmodium falciparum from Tanzania

Trans R Soc Trop Med Hyg. Nov-Dec 2002;96(6):674-6. doi: 10.1016/s0035-9203(02)90349-4.

Abstract

In 2000 we used a sensitive technique to examine 9 isolates from malaria patients in Muheza, Tanzania who had failed treatment with sulfadoxine-pyrimethamine (SP). Three isolates carried, at low levels, the leucine to isoleucine change at amino acid 164 that is associated with clinical failure of SP. Numerous other highly resistant alleles were also observed.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antimalarials / therapeutic use*
  • Child
  • Drug Resistance / genetics*
  • Gene Amplification
  • Humans
  • Malaria, Falciparum / drug therapy*
  • Plasmodium falciparum / enzymology
  • Plasmodium falciparum / genetics*
  • Point Mutation / genetics
  • Polymerase Chain Reaction / methods
  • Protozoan Proteins / genetics*
  • Pyrimethamine / therapeutic use*
  • Tanzania
  • Tetrahydrofolate Dehydrogenase / genetics*

Substances

  • Antimalarials
  • Protozoan Proteins
  • Tetrahydrofolate Dehydrogenase
  • Pyrimethamine