Expression of p53, PCNA, Ki-67 and bcl-2 in relation to risk factors in oral cancer - a molecular epidemiological study

Int J Oncol. 2003 Apr;22(4):861-8. doi: 10.3892/ijo.22.4.861.


A group of 133 primary oral squamous cell carcinomas were studied concerning a relationship between exposure factors and tumour biological parameters with a focus on the TP53 gene and p53 protein status. Tumours were evaluated using immunohistochemistry (IHC) for expression of p53, PCNA, Ki-67 and bcl-2 proteins. The TP53 gene was studied for mutations using PCR amplification of exons 5-9 and single strand conformation polymorphism (SSCP) analysis. The collected data were correlated to the exposure factors smoking, oral snuff, liquor, oral infections, dental factors, dental X-ray and iron deficiency. When compared with matched controls only oral infections, and reported HSV-infections in particular, gave statistically significant ORs (odds ratio) for all tumours (OR 8.0) as well as for the group of IHC p53 positive tumours (OR 12). No association between smoking and p53 positivity was found (OR 1.0).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carcinoma, Squamous Cell / epidemiology
  • Carcinoma, Squamous Cell / genetics
  • Carcinoma, Squamous Cell / metabolism
  • Case-Control Studies
  • Cell Division
  • Cell Line, Tumor
  • Exons
  • Genes, p53
  • Humans
  • Immunohistochemistry
  • Ki-67 Antigen / biosynthesis*
  • Mouth Neoplasms / epidemiology
  • Mouth Neoplasms / genetics*
  • Mouth Neoplasms / metabolism*
  • Mutation
  • Odds Ratio
  • Polymerase Chain Reaction
  • Polymorphism, Single-Stranded Conformational
  • Proliferating Cell Nuclear Antigen / biosynthesis*
  • Proto-Oncogene Proteins c-bcl-2 / biosynthesis*
  • Risk Factors
  • Tumor Suppressor Protein p53 / biosynthesis*
  • Tumor Suppressor Protein p53 / metabolism
  • X-Rays


  • Ki-67 Antigen
  • Proliferating Cell Nuclear Antigen
  • Proto-Oncogene Proteins c-bcl-2
  • Tumor Suppressor Protein p53