In vivo studies of liver-type fatty acid binding protein (L-FABP) gene expression in liver of transgenic zebrafish (Danio rerio)

FEBS Lett. 2003 Mar 13;538(1-3):125-33. doi: 10.1016/s0014-5793(03)00157-1.


Mammalian liver fatty acid binding protein (L-FABP) is a small cytosolic protein in various tissues including liver, small intestine and kidney and is thought to play a crucial role in intracellular fatty acid trafficking and metabolism. To better understand its tissue-specific regulation during zebrafish hepatogenesis, we isolated 5'-flanking sequences of the zebrafish L-FABP gene and used a green fluorescent protein (GFP) transgenic strategy to generate liver-specific transgenic zebrafish. The 2.8-kb 5'-flanking sequence of zebrafish L-FABP gene was sufficient to direct GFP expression in liver primordia, first observed in 2 dpf embryos and then continuously to the adult stage. This pattern of transgenic expression is consistent with the expression pattern of the endogenous gene. F2 inheritance rates of 42-51% in all the seven transgenic lines were consistent with the ratio of Mendelian segregation. Further, hhex and zXbp-1 morphants displayed a visible liver defect, which suggests that it is possible to establish an in vivo system for screening genes required for liver development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Base Sequence
  • Carrier Proteins / genetics*
  • Cloning, Molecular
  • DNA Primers
  • Fatty Acid-Binding Protein 7
  • Fatty Acid-Binding Proteins
  • Green Fluorescent Proteins
  • Liver / metabolism*
  • Luminescent Proteins / metabolism
  • Neoplasm Proteins*
  • Nerve Tissue Proteins*
  • Polymerase Chain Reaction
  • Zebrafish
  • Zebrafish Proteins*


  • Carrier Proteins
  • DNA Primers
  • Fabp1 protein, mouse
  • Fatty Acid-Binding Protein 7
  • Fatty Acid-Binding Proteins
  • Luminescent Proteins
  • Neoplasm Proteins
  • Nerve Tissue Proteins
  • Zebrafish Proteins
  • fabp7a protein, zebrafish
  • Green Fluorescent Proteins