Centrosome separation and central spindle assembly act in redundant pathways that regulate microtubule density and trigger cleavage furrow formation

Dev Cell. 2003 Mar;4(3):333-44. doi: 10.1016/s1534-5807(03)00057-1.

Abstract

The mitotic spindle provides the spatial cue that coordinates cytokinesis with nuclear division. However, the specific property of the mitotic spindle that mediates this spatial regulation remains obscure, in part because different aspects of the mitotic spindle appear to have furrow inducing activity in different systems. We show that in C. elegans embryos, although the central spindle is usually dispensable for furrow initiation, it becomes essential for furrow formation when the extent of centrosome separation during anaphase is reduced. Measurements of microtubule density demonstrate that furrow formation occurs in the vicinity of a local minimum of microtubule density. Reduction of the extent of spindle elongation or disruption of the central spindle causes delayed formation of the cleavage furrow. These data suggest that reduced microtubule density triggers cleavage furrow initiation and demonstrate that redundant mechanisms direct efficient formation of the cleavage furrow.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aurora Kinase A
  • Aurora Kinases
  • Caenorhabditis elegans / cytology
  • Caenorhabditis elegans / embryology*
  • Caenorhabditis elegans / metabolism
  • Caenorhabditis elegans Proteins / genetics
  • Caenorhabditis elegans Proteins / metabolism
  • Cell Division / physiology*
  • Centrosome / metabolism*
  • Centrosome / ultrastructure
  • Embryo, Nonmammalian / cytology
  • Embryo, Nonmammalian / embryology*
  • Embryo, Nonmammalian / metabolism
  • Kinesin / deficiency
  • Kinesin / genetics
  • Microtubules / metabolism*
  • Microtubules / ultrastructure
  • Protein-Serine-Threonine Kinases / genetics
  • Protein-Serine-Threonine Kinases / metabolism
  • Signal Transduction / physiology
  • Spindle Apparatus / metabolism*
  • Spindle Apparatus / ultrastructure
  • rhoA GTP-Binding Protein / genetics
  • rhoA GTP-Binding Protein / metabolism

Substances

  • Caenorhabditis elegans Proteins
  • ZEN-4 protein, C elegans
  • par-2 protein, C elegans
  • Aurora Kinase A
  • Aurora Kinases
  • PAR-3 protein, C elegans
  • Protein-Serine-Threonine Kinases
  • air-1 protein, C elegans
  • Kinesin
  • rhoA GTP-Binding Protein