SAS-4 is essential for centrosome duplication in C elegans and is recruited to daughter centrioles once per cell cycle

Dev Cell. 2003 Mar;4(3):431-9. doi: 10.1016/s1534-5807(03)00062-5.


The mechanisms governing centrosome duplication remain poorly understood. We identified a gene called sas-4 that is essential for this process in C. elegans. SAS-4 encodes a predicted coiled-coil protein that localizes to a tiny dot in the center of centrosomes throughout the cell cycle. FRAP experiments with GFP-SAS-4 transgenic embryos reveal that SAS-4 is recruited to the centrosome once per cell cycle, at the time of organelle duplication. Additional evidence indicates that SAS-4 is recruited to the daughter centriole or a closely associated structure. These findings identify SAS-4 recruitment as a key step in the centrosome duplication cycle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Caenorhabditis elegans / cytology
  • Caenorhabditis elegans / genetics*
  • Caenorhabditis elegans / metabolism*
  • Caenorhabditis elegans Proteins / genetics*
  • Caenorhabditis elegans Proteins / metabolism*
  • Cell Cycle / genetics*
  • Centrioles / genetics
  • Centrioles / metabolism
  • Centrosome / metabolism*
  • Embryo, Nonmammalian / cytology
  • Embryo, Nonmammalian / metabolism
  • Female
  • Fluorescence Resonance Energy Transfer
  • Fluorescent Antibody Technique
  • Gene Expression Regulation / genetics
  • Germ Cells / cytology
  • Germ Cells / metabolism
  • Green Fluorescent Proteins
  • Luminescent Proteins
  • Male
  • Molecular Sequence Data
  • Protein Kinases / deficiency
  • Protein Kinases / genetics
  • RNA Interference / physiology
  • Spermatozoa / cytology
  • Spermatozoa / metabolism


  • Caenorhabditis elegans Proteins
  • Luminescent Proteins
  • SAS-4 protein, C elegans
  • Green Fluorescent Proteins
  • Protein Kinases
  • zyg-1 protein, C elegans

Associated data

  • GENBANK/AJ539470