Amniotic fluid levels of S100B protein in normal and trisomy-21 foetuses

Clin Chim Acta. 2003 Apr;330(1-2):131-3. doi: 10.1016/s0009-8981(03)00010-x.

Abstract

Background: The human chromosome 21 has been shown to contain the gene for the beta subunit of the S100B protein. The present case-control study was aimed at investigating whether overproduction of S100B protein is detectable in the amniotic fluid of foetuses with trisomy-21.

Methods: Measurements of S100B in amniotic fluid samples from 14 pregnant women with trisomy-21 foetuses were compared with those obtained from 182 physiological pregnancies. S100B was measured in the samples using an immunoluminometric assay (LIA-mat Sangtec 100).

Results: Our results showed that S100B protein amniotic fluid levels were significantly higher in trisomy-21 foetuses (0.83+/-0.21 microg/l) than in controls (0.51+/-0.22 microg/l) (p<0.05).

Conclusion: The present finding supports the notion that the expression of S100B is increased in trisomy-21 foetuses; it also constitutes a prerequisite basis for a possible involvement of the protein in pathogenic processes associated with trisomy-21, and/or for its potential employment as a diagnostic tool.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Amniotic Fluid / metabolism*
  • Autoantigens / analysis
  • Case-Control Studies
  • Chromosomes, Human, Pair 21 / metabolism*
  • Down Syndrome / metabolism*
  • Female
  • Fetus / metabolism*
  • Humans
  • Immunoassay / methods
  • Luminescent Measurements
  • Male
  • Nerve Growth Factors / metabolism*
  • Pregnancy
  • S100 Calcium Binding Protein beta Subunit
  • S100 Proteins / metabolism*

Substances

  • Autoantigens
  • Nerve Growth Factors
  • S100 Calcium Binding Protein beta Subunit
  • S100 Proteins
  • S100B protein, human