Extended-spectrum beta-lactamase types in Klebsiella pneumoniae and Escherichia coli in two Greek hospitals

Int J Antimicrob Agents. 2003 Mar;21(3):285-8. doi: 10.1016/s0924-8579(02)00361-8.

Abstract

Seventy-nine Klebsiella pneumoniae and 124 Escherichia coli clinical strains, isolated consecutively during August-October 2001 in two Greek hospitals, were examined for production of extended-spectrum beta-lactamases (ESBLs). Seventy-one (35%) isolates (46 K. pneumoniae and 25 E. coli) were ESBL-positive by phenotypic methods. Isoelectric focusing of beta-lactamases and PCR assays for bla genes showed that SHV-5-type ESBLs were the most frequent (45 isolates, 22%) followed by CTX-M (24 isolates, 12%) and IBC (three isolates, 1.5%). The latter two ESBL types may have been established recently in this setting.

MeSH terms

  • Base Sequence
  • DNA, Bacterial / genetics
  • Drug Resistance, Bacterial / genetics
  • Escherichia coli / enzymology*
  • Escherichia coli / isolation & purification
  • Escherichia coli Infections / drug therapy
  • Escherichia coli Infections / epidemiology
  • Escherichia coli Infections / microbiology
  • Genes, Bacterial
  • Greece / epidemiology
  • Humans
  • In Vitro Techniques
  • Isoelectric Focusing
  • Klebsiella Infections / drug therapy
  • Klebsiella Infections / epidemiology
  • Klebsiella Infections / microbiology
  • Klebsiella pneumoniae / drug effects
  • Klebsiella pneumoniae / enzymology*
  • Klebsiella pneumoniae / genetics
  • Klebsiella pneumoniae / isolation & purification
  • Polymerase Chain Reaction
  • beta-Lactamases / biosynthesis*
  • beta-Lactamases / classification
  • beta-Lactamases / drug effects
  • beta-Lactamases / genetics
  • beta-Lactamases / isolation & purification

Substances

  • DNA, Bacterial
  • beta-Lactamases