Bifidobacterium-fermented soy milk extract stimulates hyaluronic acid production in human skin cells and hairless mouse skin

Skin Pharmacol Appl Skin Physiol. 2003 Mar-Apr;16(2):108-16. doi: 10.1159/000069031.


We examined the effects of Bifidobasterium-fermented (BE) and nonfermented (SME) soy milk extracts on the production of hyaluronic acid (HA) in vitro and in vivo. BE, but not SME, significantly enhanced the production of HA in monolayer and organotypic cultures of human keratinocytes, in cultures of human skin fibroblasts, and in hairless mouse skin following topical application for 2 weeks. In the organotypic cultures formed by a similar structure to human epidermis, BE also extended the distribution of HA. Genistein and daidzein, known to stimulate HA production, were detected in BE at a concentration of 0.18 and 0.07 mM, respectively, but not in SME. Therefore, BE has the potential to enhance HA production in the epidermis and dermis, mainly due to genistein released from its glycoside during fermentation. BE is expected to prevent the age-dependent loss of cutaneous HA.

Publication types

  • Comparative Study

MeSH terms

  • Administration, Cutaneous
  • Animals
  • Bifidobacterium*
  • Culture Techniques
  • Fermentation
  • Fibroblasts / drug effects
  • Fibroblasts / metabolism
  • Humans
  • Hyaluronic Acid / biosynthesis*
  • Keratinocytes / drug effects
  • Keratinocytes / metabolism
  • Male
  • Mice
  • Mice, Hairless
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology
  • Skin / cytology
  • Skin / drug effects*
  • Skin / metabolism
  • Soybeans / chemistry*
  • Species Specificity


  • Plant Extracts
  • Hyaluronic Acid