BCL-2/JH translocation in peripheral blood lymphocytes of unexposed individuals: lack of seasonal variations in frequency and molecular features

Int J Cancer. 2003 May 10;104(6):695-8. doi: 10.1002/ijc.10975.


BCL-2/J(H) rearrangement has been proposed as a biomarker for evaluating the genotoxicity of occupational and environmental exposures. Available data on time-related modification of this rearrangement in peripheral blood lymphocytes in unexposed healthy individuals is scarce. We investigated the characteristics of BCL-2/J(H) rearrangements in 33 adults unexposed to genotoxins at 2 seasonal time points: winter and spring. BCL-2/J(H) rearrangement was detected in 79% of individuals (detection limit = 8.48 x 10(-8)). Its frequency ranged from <1 to 40 translocations per million lymphocytes with a significant (p = 0.04) positive correlation with age. No significant modifications of BCL-2/J(H) rearrangement frequency or in the number of clones harboring this rearrangement were observed according the 2 time points. No obvious influence of season-related environmental factors on frequency or molecular features of BCL-2/J(H) rearrangements was found in this population suggesting that this would not be a confounding factor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Chromosome Aberrations
  • Chromosomes, Human, Pair 14 / genetics
  • Chromosomes, Human, Pair 18 / genetics
  • DNA / analysis
  • DNA Primers
  • Flow Cytometry
  • Gene Frequency
  • Gene Rearrangement*
  • Humans
  • Immunoglobulin Joining Region / genetics*
  • Lymphocytes / blood*
  • Male
  • Middle Aged
  • Polymerase Chain Reaction
  • Proto-Oncogene Proteins c-bcl-2 / genetics*
  • Seasons
  • Translocation, Genetic / genetics*


  • DNA Primers
  • Immunoglobulin Joining Region
  • Proto-Oncogene Proteins c-bcl-2
  • DNA