Cloning, expression, and genomic structure of a novel human Rap2 interacting gene (RPIP9)

Biochem Genet. 2003 Feb;41(1-2):13-25. doi: 10.1023/a:1020918411532.

Abstract

During a large-scale screen of a human fetal brain cDNA library, a full-length cDNA encoding a novel Rap2 interacting protein was isolated and sequenced. The cDNA is 3397bp long and has a predicted open reading frame encoding a protein of 329 aa. The predicted protein shows high homology to mouse and human RPIP8, and has a RUN domain near its C-terminus. The gene was mapped to human chromosome 7q21-7q22 and has 9 exons and 8 introns. The expression pattern was also detected by cycle-limited reverse polymerase chain reaction (RT-PCR).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism*
  • Chromosomes, Human, Pair 7*
  • Cloning, Molecular
  • DNA, Complementary
  • Gene Expression
  • Humans
  • Intracellular Signaling Peptides and Proteins
  • Molecular Sequence Data
  • Nerve Tissue Proteins / genetics*
  • Nerve Tissue Proteins / metabolism*
  • Organ Specificity
  • Physical Chromosome Mapping
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • rap GTP-Binding Proteins / metabolism*

Substances

  • Carrier Proteins
  • DNA, Complementary
  • Intracellular Signaling Peptides and Proteins
  • Nerve Tissue Proteins
  • RUNDC3B protein, human
  • RAP2A protein, human
  • Rap2a protein, mouse
  • rap GTP-Binding Proteins

Associated data

  • GENBANK/AY033596