Androgen-independent growth in LNCaP cell lines and steroid uridine diphosphate-glucuronosyltransferase expression

Asian J Androl. 2003 Mar;5(1):9-13.


Aim: To investigate the mechanism of androgen-independent growth of prostate cancer after androgen ablation in LNCaP cells and the effect of glucuronidation activity.

Methods: To establish androgen-independent growth in prostate cancer LNCaP-SF, continuous passage was performed in androgen-stripped medium and the cells were evaluated for glucuronidation activity. The expression vector of antisense uridine diphosphate glucuronosyl-transferase (UGT) 2B15 cDNA was also constructed and evaluated.

Results: LNCaP-SF lead to a higher expression in UGT2B15 and their glucuronidation activity is 2.5 times higher than that of LNCaP cells. Significantly fewer LNCaP and LNCaP-SF than control were transfected with the antisense UGT2B15 cDNA, suggesting that UGT2B15 plays an important part in the glucuronidation activity of androgens in both cells.

Conclusion: The alteration of UGT2B15 expression in LNCaP-SF cells is proposed as a biological characteristic involved in the growth of hormone-refractory prostate cancer.

MeSH terms

  • Androgens / metabolism*
  • Cell Division / physiology
  • DNA, Antisense
  • Glucuronic Acid / metabolism*
  • Glucuronosyltransferase / genetics*
  • Glucuronosyltransferase / metabolism*
  • Humans
  • Male
  • Prostatic Neoplasms*
  • Transfection
  • Tumor Cells, Cultured / cytology
  • Tumor Cells, Cultured / enzymology


  • Androgens
  • DNA, Antisense
  • Glucuronic Acid
  • Glucuronosyltransferase
  • UDP-glucuronosyltransferase 2B15, human