Isolation and identification of four flavonoid constituents from the seeds of Oroxylum indicum by high-speed counter-current chromatography

J Chromatogr A. 2003 Feb 21;988(1):95-105. doi: 10.1016/s0021-9673(02)01954-4.

Abstract

Four flavonoids, chrysin, baicalein, baicalein-7-O-glucoside, baicalein-7-O-diglucoside (Oroxylin B) and one unknown flavonoid have been isolated and purified for the first time in the seeds of Oroxylum indicum by high-speed counter-current chromatography with a two-phase solvent system composed of chloroform-methanol-water (8:10:5, v/v). Then, 50 mg baicalein-7-O-glucoside, 10.5 mg baicalein-7-O-diglucoside, 4.5 mg chrysin-7-O-diglucoside, 25 mg baicalein and 45 mg chrysin could be obtained after injecting 20 mg/ml sample extract ten times and their purities were 96, 90, 85, 95 and 98%, respectively. All these constituents were identified by high-performance liquid chromatography-mass spectrometry and nuclear magnetic resonance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bignoniaceae / chemistry*
  • Countercurrent Distribution / methods*
  • Flavonoids / chemistry
  • Flavonoids / isolation & purification*
  • Magnetic Resonance Spectroscopy
  • Seeds / chemistry*

Substances

  • Flavonoids