Prognostic Significance of CD38 and CD20 Expression as Assessed by Quantitative Flow Cytometry in Chronic Lymphocytic Leukaemia

Br J Haematol. 2003 Mar;120(6):1017-25. doi: 10.1046/j.1365-2141.2003.04213.x.

Abstract

CD38 expression on chronic lymphocytic leukaemia (CLL) cells is a poor prognostic factor, however, methods for measuring this vary. The QuantiBRITETM flow cytometry (FC) system yields an absolute antigen expression value (antibodies bound/cell, ABC) and may be useful in standardizing CD38 expression analysis. We evaluated cryopreserved pretreatment CLL cells for CD20 ABC, CD38 ABC, and percentage of CD38+ B cells from 131 patients requiring therapy. The 92 patients (70%) with >/= 100 CD38 ABC had worse overall survival (OS; 34% at 5 years) compared with those with < 100 CD38 ABC (70% at 5 years, mortality hazard ratio 2.30, 95% confidence interval 1.28-4.12; two-tailed P = 0.003). Among the 64 patients with < 30% CD38+ cells, OS of the 25 with >/= 100 ABC was worse than that of the 39 with < 100 ABC (P = 0.018). OS of patients with < 30% CD38+ cells and >/= 100 ABC was actually similar to that of patients with >/= 30% CD38+ cells. BrightCD20 expression (>/= 20 000 ABC) was not associated with a worse OS (P = 0.10). The presence of >/= 100 CD38 ABC in CLL patients requiring therapy is an unfavourable prognostic factor for OS and quantitative FC may be superior to percentage CD38+ cell assessment. Prospective trials are required to determine more precisely the prognostic significance of absolute expression levels in fresh CLL cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • ADP-ribosyl Cyclase / analysis*
  • ADP-ribosyl Cyclase 1
  • Adult
  • Aged
  • Aged, 80 and over
  • Antigens, CD / analysis*
  • Antigens, CD20 / analysis*
  • B-Lymphocytes / immunology
  • Biomarkers / analysis
  • Cells, Cultured
  • Cryopreservation
  • Female
  • Flow Cytometry
  • Humans
  • Leukemia, Lymphocytic, Chronic, B-Cell / immunology*
  • Leukemia, Lymphocytic, Chronic, B-Cell / mortality
  • Lymphocyte Count
  • Male
  • Membrane Glycoproteins
  • Middle Aged
  • Prognosis
  • Survival Rate

Substances

  • Antigens, CD
  • Antigens, CD20
  • Biomarkers
  • Membrane Glycoproteins
  • ADP-ribosyl Cyclase
  • CD38 protein, human
  • ADP-ribosyl Cyclase 1