Global approaches to protein-protein interactions

Curr Opin Cell Biol. 2003 Apr;15(2):199-205. doi: 10.1016/s0955-0674(03)00005-x.


The availability of complete, annotated genome sequences for a variety of eukaryotic organisms has paved the way for a paradigm shift in biomedical research from the 'one gene-one hypothesis' approach to more global, systematic strategies that analyse genes or proteins on a genome- and proteome-wide scale. One daunting task in the post-genome era is to determine how the complement of expressed cellular proteins - the proteome - is organised into functional, higher-order networks, by mapping all constitutive and dynamic protein-protein interactions. Traditionally, reductionist approaches have typically focused on a few, selected gene products and their interactions in a particular physiological context. In contrast, more holistic strategies aim at understanding complex biological systems, for example global protein-protein interaction networks on a cellular or organismal level. Several large-scale proteomics technologies have been developed to generate comprehensive, cellular protein-protein interaction maps.

Publication types

  • Review

MeSH terms

  • Eukaryotic Cells / metabolism*
  • Fungal Proteins / metabolism
  • Models, Biological
  • Proteins / genetics
  • Proteins / metabolism*
  • Proteomics / methods*
  • Proteomics / trends
  • Yeasts / metabolism*


  • Fungal Proteins
  • Proteins