Prostaglandin F(2alpha) stimulation of cyclooxygenase-2 promoter activity by the FP(B) prostanoid receptor

Eur J Pharmacol. 2003 Mar 28;465(1-2):39-41. doi: 10.1016/s0014-2999(03)01485-7.

Abstract

We have recently shown that the FP(B) prostanoid receptor activates beta-catenin signaling through the activation of Rho in human embryo kidney (HEK)-293 cells stably expressing the FP(B) receptors. We now report that the FP(B) receptor can stimulate cyclooxygenase-2 promoter activity and may, therefore, regulate the expression of cyclooxygenase-2. This stimulation of cyclooxygenase-2 promoter activity is blocked by pretreatment with an inhibitor of Rho, but not with an inhibitor of protein kinase C (PKC). Potential up regulation of cyclooxygenase-2 expression by the FP(B) receptor would establish a positive feedback loop that would drive beta-catenin signaling and could be involved in cancer.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • ADP Ribose Transferases / pharmacology
  • Animals
  • Botulinum Toxins / pharmacology
  • Cell Line
  • Cyclooxygenase 2
  • Dinoprost / pharmacology*
  • Enzyme Inhibitors / pharmacology
  • Gene Expression Regulation, Enzymologic / drug effects
  • Humans
  • Indoles / pharmacology
  • Isoenzymes / genetics*
  • Luciferases / genetics
  • Luciferases / metabolism
  • Maleimides / pharmacology
  • Membrane Proteins
  • Promoter Regions, Genetic / genetics*
  • Prostaglandin-Endoperoxide Synthases / genetics*
  • Protein Isoforms / genetics
  • Protein Isoforms / physiology
  • Protein Kinase C / antagonists & inhibitors
  • Receptors, Prostaglandin / genetics
  • Receptors, Prostaglandin / physiology*
  • Recombinant Fusion Proteins / drug effects
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Swine
  • Transfection
  • rho GTP-Binding Proteins / antagonists & inhibitors

Substances

  • Enzyme Inhibitors
  • Indoles
  • Isoenzymes
  • Maleimides
  • Membrane Proteins
  • Protein Isoforms
  • Receptors, Prostaglandin
  • Recombinant Fusion Proteins
  • Dinoprost
  • Luciferases
  • Cyclooxygenase 2
  • PTGS2 protein, human
  • Prostaglandin-Endoperoxide Synthases
  • ADP Ribose Transferases
  • exoenzyme C3, Clostridium botulinum
  • Protein Kinase C
  • Botulinum Toxins
  • rho GTP-Binding Proteins
  • bisindolylmaleimide