GABAA receptor epsilon subunit expression in identified peptidergic neurons of the rat hypothalamus

Brain Res. 2003 Mar 28;967(1-2):285-9. doi: 10.1016/s0006-8993(02)04270-1.

Abstract

Dual-labeling immunohistochemical or in situ hybridization studies for the recently cloned epsilon-subunit and several neuropeptides were performed in the rat hypothalamus. We revealed an extensive co-expression (>90%) with hypocretin (Hcrt), oxytocin (OT), the gonadotropin-releasing hormone (GnRH), and the melanin-concentrating hormone (MCH) peptides, whereas occasional co-expression (<10%) with cocaine-amphetamine-regulated transcript (CART) was found. Our results suggest that novel GABA(A) receptor subtypes comprising epsilon-subunit are important for metabolic and neuroendocrine functions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Hypothalamus / chemistry
  • Hypothalamus / metabolism*
  • Male
  • Nerve Tissue Proteins / analysis
  • Nerve Tissue Proteins / biosynthesis*
  • Neurons / chemistry
  • Neurons / metabolism*
  • Peptides / analysis
  • Peptides / metabolism
  • Protein Subunits / analysis
  • Protein Subunits / biosynthesis*
  • Rats
  • Rats, Wistar
  • Receptors, GABA-A / analysis
  • Receptors, GABA-A / biosynthesis*

Substances

  • Gabre protein, rat
  • Nerve Tissue Proteins
  • Peptides
  • Protein Subunits
  • Receptors, GABA-A