Structure of the main ganglioside from the brain of Xenopus laevis

Glycoconj J. 2002 Jan;19(1):53-7. doi: 10.1023/a:1022584914778.

Abstract

The main component of the ganglioside(1) mixture from the brain of the adult amphibian Xenopus laevis accounts for 35% of the total, as lipid bound sialic acid. This ganglioside has been purified and characterized by thin layer chromatography, partial and exhaustive enzymatic hydrolysis with sialidase, TLC-overlay procedures with anti-Gg(4)Cer and anti-Neu5Acalpha6GalNAcbeta specific monoclonal antibodies and mass spectrometry. All together the results suggest the following structure: Neu5Acalpha8Neu5Acalpha3Galbeta3(Neu5Acalpha8Neu5Acalpha6)GalNAcbeta4Galbeta4Glcbeta1Cer, or, IV(3)-alpha-Neu5Ac(2),III(6)-alpha-Neu5Ac(2)-Gg(4)Cer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain Chemistry*
  • Carbohydrate Sequence
  • Chromatography, High Pressure Liquid
  • Chromatography, Thin Layer
  • Gangliosides / chemistry*
  • Gangliosides / metabolism
  • Mass Spectrometry
  • Molecular Sequence Data
  • Neuraminidase / metabolism
  • Xenopus laevis*

Substances

  • Gangliosides
  • Neuraminidase