Erosion of the telomeric single-strand overhang at replicative senescence

Nat Genet. 2003 Apr;33(4):492-6. doi: 10.1038/ng1127. Epub 2003 Mar 24.


Cultured primary human cells inevitably enter a state of replicative senescence for which the specific molecular trigger is unknown. We show that the single-strand telomeric overhang, a key component of telomere structure, is eroded at senescence. Expression of telomerase prevents overhang loss, suggesting that this enzyme prevents senescence by maintaining proper telomere structure. In contrast, progressive overhang loss occurs in cells that avoid senescence through the inactivation of p53 and Rb, indicating that overhang erosion is the result of continuous cell division and not a consequence of senescence. We thus provide evidence for a specific molecular alteration in telomere structure at senescence and suggest that this change, rather than overall telomere length, serves to trigger this state.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antigens, Viral, Tumor / metabolism
  • Blotting, Southern
  • Cell Division
  • Cells, Cultured
  • Cellular Senescence / physiology*
  • Densitometry
  • Fibroblasts / metabolism
  • Genetic Techniques
  • Humans
  • Oxidative Stress / physiology
  • Protein Synthesis Inhibitors / pharmacology
  • Retinoblastoma Protein / metabolism
  • Telomere / metabolism*
  • Telomere / physiology*
  • Time Factors
  • Tumor Suppressor Protein p53 / metabolism


  • Antigens, Viral, Tumor
  • Protein Synthesis Inhibitors
  • Retinoblastoma Protein
  • Tumor Suppressor Protein p53