Amplifying whole insect genomes with multiple displacement amplification

Insect Mol Biol. 2003 Apr;12(2):195-200. doi: 10.1046/j.1365-2583.2003.00401.x.


Many applications in insect genetics require repeated analyses on individual organisms. These include population genetic and genomics, linkage mapping, molecular systematics and map-based positional cloning. However, using the polymerase chain reaction, a limited number of analyses are possible with DNA isolated from whole bodies or parts of small or preserved specimens. We describe the optimization of a new technique, Multiple Displacement Amplification, for 100-400-fold amplification of whole mosquito genomes. We demonstrate that MDA amplifies genomic DNA directly from an adult leg or from organisms as small as a first instar mosquito larva. Genetic polymorphisms revealed at individual loci are the same whether using the original genomic DNA or MDA DNA as a template.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aedes / genetics*
  • Animals
  • DNA / analysis
  • DNA / genetics
  • Female
  • Genes, Insect / genetics*
  • Genetic Techniques
  • Genome
  • Polymerase Chain Reaction / methods*
  • Polymorphism, Genetic
  • Polymorphism, Single-Stranded Conformational


  • DNA