ANKTM1, a TRP-like Channel Expressed in Nociceptive Neurons, Is Activated by Cold Temperatures

Cell. 2003 Mar 21;112(6):819-29. doi: 10.1016/s0092-8674(03)00158-2.

Abstract

Mammals detect temperature with specialized neurons in the peripheral nervous system. Four TRPV-class channels have been implicated in sensing heat, and one TRPM-class channel in sensing cold. The combined range of temperatures that activate these channels covers a majority of the relevant physiological spectrum sensed by most mammals, with a significant gap in the noxious cold range. Here, we describe the characterization of ANKTM1, a cold-activated channel with a lower activation temperature compared to the cold and menthol receptor, TRPM8. ANKTM1 is a distant family member of TRP channels with very little amino acid similarity to TRPM8. It is found in a subset of nociceptive sensory neurons where it is coexpressed with TRPV1/VR1 (the capsaicin/heat receptor) but not TRPM8. Consistent with the expression of ANKTM1, we identify noxious cold-sensitive sensory neurons that also respond to capsaicin but not to menthol.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Ankyrins / chemistry
  • CHO Cells
  • Calcium Channels / metabolism*
  • Capsaicin / pharmacology
  • Cells, Cultured
  • Cold Temperature*
  • Cricetinae
  • Female
  • Membrane Proteins / chemistry
  • Mice
  • Molecular Sequence Data
  • Nerve Tissue Proteins / metabolism
  • Neurons, Afferent / metabolism*
  • Nociceptors / metabolism*
  • Protein Structure, Tertiary
  • Rats
  • Rats, Sprague-Dawley
  • TRPA1 Cation Channel
  • TRPC Cation Channels
  • Thermoreceptors / chemistry
  • Thermoreceptors / metabolism*
  • Transient Receptor Potential Channels / metabolism*

Substances

  • Ankyrins
  • Calcium Channels
  • Membrane Proteins
  • Nerve Tissue Proteins
  • TRPA1 Cation Channel
  • TRPA1 protein, human
  • TRPC Cation Channels
  • Transient Receptor Potential Channels
  • Capsaicin

Associated data

  • GENBANK/AY231177