Multiplex PCR for detection of aminoglycoside resistance genes in enterococci

Antimicrob Agents Chemother. 2003 Apr;47(4):1423-6. doi: 10.1128/AAC.47.4.1423-1426.2003.

Abstract

A multiplex PCR procedure for detecting the aminoglycoside resistance genes aac(6')-Ie-aph(2")-Ia, aph(2")-Ib, aph(2")-Ic, aph(2")-Id, aph(3')-IIIa, and ant(4')-Ia was evaluated and found to determine accurately the presence of these genes in enterococci.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Aminoglycosides
  • Animals
  • Anti-Bacterial Agents / pharmacology*
  • Drug Resistance, Bacterial / genetics*
  • Enterococcus / drug effects*
  • Enterococcus / genetics*
  • Humans
  • Polymerase Chain Reaction / methods*

Substances

  • Aminoglycosides
  • Anti-Bacterial Agents