Multiplex PCR for detection of aminoglycoside resistance genes in enterococci

Sergei B Vakulenko; Susan M Donabedian; Anatoliy M Voskresenskiy; Marcus J Zervos; Stephen A Lerner; Joseph W Chow

doi:10.1128/AAC.47.4.1423-1426.2003

Multiplex PCR for detection of aminoglycoside resistance genes in enterococci

Antimicrob Agents Chemother. 2003 Apr;47(4):1423-6. doi: 10.1128/AAC.47.4.1423-1426.2003.

Authors

Sergei B Vakulenko¹, Susan M Donabedian, Anatoliy M Voskresenskiy, Marcus J Zervos, Stephen A Lerner, Joseph W Chow

Affiliation

¹ Division of Infectious Diseases, Department of Biochemistry and Molecular Biology, Wayne State University, Detroit, Michigan 48201, USA.

Abstract

A multiplex PCR procedure for detecting the aminoglycoside resistance genes aac(6')-Ie-aph(2")-Ia, aph(2")-Ib, aph(2")-Ic, aph(2")-Id, aph(3')-IIIa, and ant(4')-Ia was evaluated and found to determine accurately the presence of these genes in enterococci.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Aminoglycosides
Animals
Anti-Bacterial Agents / pharmacology*
Drug Resistance, Bacterial / genetics*
Enterococcus / drug effects*
Enterococcus / genetics*
Humans
Polymerase Chain Reaction / methods*

Substances

Aminoglycosides
Anti-Bacterial Agents