Chimeric 16S rDNA sequences of diverse origin are accumulating in the public databases

Int J Syst Evol Microbiol. 2003 Jan;53(Pt 1):289-293. doi: 10.1099/ijs.0.02441-0.


A significant number of chimeric 16S rDNA sequences of diverse origin were identified in the public databases by partial treeing analysis. This suggests that chimeric sequences, representing phylogenetically novel non-existent organisms, are routinely being overlooked in molecular phylogenetic surveys despite a general awareness of PCR-generated artefacts amongst researchers.

MeSH terms

  • Chimera / genetics*
  • DNA, Bacterial / genetics*
  • DNA, Ribosomal / genetics*
  • Databases, Nucleic Acid*
  • Phylogeny
  • RNA, Bacterial / genetics
  • RNA, Ribosomal, 16S / genetics


  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Bacterial
  • RNA, Ribosomal, 16S