Technical Note: Specific PCR Amplification of Protozoal 18S rDNA Sequences From DNA Extracted From Ruminal Samples of Cows

J Anim Sci. 2003 Mar;81(3):812-5. doi: 10.2527/2003.813812x.

Abstract

A protozoa-specific primer (P-SSU-342f) was designed and paired with a eukarya-specific primer to amplify a 1,360-bp fragment of DNA encoding protozoal small subunit (SSU) ribosomal RNA from ruminal fluid of cows fed a mixed forage:grain diet or alfalfa hay. Sequencing of clones showed that P-SSU-342f is specific to ruminal protozoa and, with slight modifications, the primer will be useful for ecological studies of ruminal protozoa.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Cattle
  • DNA Primers
  • DNA, Protozoan / genetics*
  • DNA, Ribosomal / genetics*
  • Eukaryota / classification*
  • Eukaryota / genetics
  • Gene Amplification
  • Molecular Sequence Data
  • Phylogeny
  • Polymerase Chain Reaction / veterinary
  • RNA, Ribosomal, 18S / genetics*
  • Rumen / parasitology*
  • Sequence Alignment / veterinary

Substances

  • DNA Primers
  • DNA, Protozoan
  • DNA, Ribosomal
  • RNA, Ribosomal, 18S