Phospholipase C is required for the control of stomatal aperture by ABA

Plant J. 2003 Apr;34(1):47-55. doi: 10.1046/j.1365-313x.2003.01698.x.

Abstract

The calcium-releasing second messenger inositol 1,4,5-trisphosphate is involved in the regulation of stomatal aperture by ABA. In other signalling pathways, inositol 1,4,5-trisphosphate is generated by the action of phospholipase C. We have studied the importance of phospholipase C in guard cell ABA-signalling pathways. Immunolocalisation of a calcium-activated phospholipase C confirmed the presence of phospholipase C in tobacco guard cells. Transgenic tobacco plants with considerably reduced levels of phospholipase C in their guard cells were only partially able to regulate their stomatal apertures in response to ABA. These results suggest that phospholipase C is involved in the amplification of the calcium signal responsible for reductions in stomatal aperture in response to ABA. As full ABA-induced inhibition of stomatal opening was not observed, our results support a role for the action of other calcium-releasing second messengers in the guard cell ABA-signalling pathway. It is not known whether these different calcium-releasing second messengers act in the same or parallel ABA-signalling pathways.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abscisic Acid / pharmacology*
  • Calcium / metabolism
  • Disasters
  • Gene Expression
  • Plant Leaves / cytology*
  • Plant Leaves / enzymology*
  • Plant Leaves / genetics
  • Plants, Genetically Modified
  • Signal Transduction / drug effects*
  • Tobacco / cytology*
  • Tobacco / enzymology*
  • Tobacco / genetics
  • Type C Phospholipases / genetics
  • Type C Phospholipases / metabolism*

Substances

  • Abscisic Acid
  • Type C Phospholipases
  • Calcium