Chromatin Immunoprecipitation Reveals That the 180-bp Satellite Repeat Is the Key Functional DNA Element of Arabidopsis Thaliana Centromeres

Genetics. 2003 Mar;163(3):1221-5.

Abstract

The centromeres of Arabidopsis thaliana chromosomes contain megabases of complex DNA consisting of numerous types of repetitive DNA elements. We developed a chromatin immunoprecipitation (ChIP) technique using an antibody against the centromeric H3 histone, HTR12, in Arabidopsis. ChIP assays showed that the 180-bp centromeric satellite repeat was precipitated with the antibody, suggesting that this repeat is the key component of the centromere/kinetochore complex in Arabidopsis.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Arabidopsis / genetics*
  • Arabidopsis Proteins / genetics
  • Centromere / genetics*
  • Chromatin / isolation & purification*
  • Cloning, Molecular
  • DNA, Plant / genetics
  • Microsatellite Repeats / genetics*

Substances

  • Arabidopsis Proteins
  • Chromatin
  • DNA, Plant