C-terminal domain of a hevein-like protein from Wasabia japonica has potent antimicrobial activity

Plant Cell Physiol. 2003 Mar;44(3):296-303. doi: 10.1093/pcp/pcg035.

Abstract

An antimicrobial protein, designated WjAMP-1, was purified from leaves of Wasabia japonica L. WjAMP-1 showed antimicrobial activity against both fungi and bacteria. The deduced amino acid sequence of cDNA of WjAMP-1 showed 60% and 70% identity with a hevein from Hevea brasiliansis and a hevein-like protein from Arabidopsis thaliana, respectively. However, matured WjAMP-1 lacked the hevein domain and may correspond to the C-terminal domain of hevein. Southern blot analysis showed that one or two copies of the WjAMP-1 gene were presented in the genome of wasabi. Expression of WjAMP-1 was detected in all organs tested, and was especially strong in petioles. Expression of WjAMP-1 was induced by the inoculation with fungal pathogens and treatment with methyl jasmonate. Recombinant WjAMP-1 expressed in Nicotiana benthamiana using potato virus X vector also inhibited not only growth of fungi but also bacteria. These results suggest that WjAMP-1 may be the C-terminal domain of hevein and one of the defense gene in W. japonica. WjAMP-1 gene may be useful genes to generate resistant plants against fungal and bacterial pathogens.

MeSH terms

  • Alternaria / drug effects
  • Amino Acid Sequence
  • Antimicrobial Cationic Peptides / pharmacology*
  • Bacteria / drug effects*
  • Base Sequence
  • Botrytis / drug effects
  • Cloning, Molecular
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • Fungi / drug effects*
  • Fusarium / drug effects
  • Gene Expression Regulation, Plant
  • Magnaporthe / drug effects
  • Molecular Sequence Data
  • Multigene Family / genetics
  • Plant Lectins / chemistry
  • Plant Lectins / genetics*
  • Plant Lectins / isolation & purification
  • Plant Lectins / metabolism*
  • Plant Lectins / pharmacology
  • Sequence Analysis, DNA
  • Wasabia / genetics
  • Wasabia / metabolism*

Substances

  • Antimicrobial Cationic Peptides
  • DNA, Complementary
  • Plant Lectins
  • hevein

Associated data

  • GENBANK/AB091697