Protective effect of three diallyl sulphides against glucose-induced erythrocyte and platelet oxidation, and ADP-induced platelet aggregation

Thromb Res. 2002 Dec 15;108(5-6):317-22. doi: 10.1016/s0049-3848(03)00106-3.


Isolated human erythrocyte membrane and platelet were used to study the antioxidative and anti-aggregative effects of three diallyl sulphides (diallyl sulphide, DAS; diallyl disulphide, DADS; diallyl trisulphide, DAT) against glucose-induced oxidation and adenosine 5'-diphosphate (ADP)-induced platelet aggregation. Three sulphide agents showed dose-dependent antioxidative protection against glucose-induced erythrocyte membrane oxidation (p<.05), and these agents at 10 microM significantly increased the retention of alpha-tocopherol in erythrocyte membrane (p<.05), in which DAT was the most effective agent (p<.05). Three sulphide agents significantly inhibited 30 and 50 mM of glucose-induced platelet oxidation (p<.05). The anti-aggregative activity of each sulphide agent was dose dependent (p<.05), in which DAT showed the greatest inhibitory effect on platelet aggregation than DADS, followed by DAS (p<.05). After ADP stimulation, the malondialdehyde (MDA) formation in platelets treated with sulphide agents was significantly less (p<.05), in which DADS and DAT showed similar antioxidative activities (p>.05). These results suggested that DADS and DAT could be considered as strong antioxidative and antithrombotic agents to prevent or control oxidative damage and platelet hyperactivity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Diphosphate / pharmacology
  • Adult
  • Allyl Compounds / pharmacology*
  • Antioxidants / pharmacology*
  • Blood Platelets / drug effects*
  • Blood Platelets / metabolism
  • Blood Platelets / physiology
  • Disulfides / pharmacology
  • Drug Antagonism
  • Erythrocytes / drug effects*
  • Erythrocytes / metabolism
  • Glucose / pharmacology*
  • Humans
  • Malondialdehyde / metabolism
  • Oxidation-Reduction / drug effects
  • Platelet Aggregation / drug effects
  • Sulfides / pharmacology*
  • alpha-Tocopherol / metabolism


  • Allyl Compounds
  • Antioxidants
  • Disulfides
  • Sulfides
  • diallyl trisulfide
  • Malondialdehyde
  • diallyl disulfide
  • allyl sulfide
  • Adenosine Diphosphate
  • alpha-Tocopherol
  • Glucose