The Role of Ppt/Wnt5 in Regulating Cell Shape and Movement During Zebrafish Gastrulation

Mech Dev. 2003 Apr;120(4):467-76. doi: 10.1016/s0925-4773(03)00004-2.

Abstract

Wnt genes play important roles in regulating patterning and morphogenesis during vertebrate gastrulation. In zebrafish, slb/wnt11 is required for convergence and extension movements, but not cell fate specification during gastrulation. To determine if other Wnt genes functionally interact with slb/wnt11, we analysed the role of ppt/wnt5 during zebrafish gastrulation. ppt/wnt5 is maternally provided and zygotically expressed at all stages during gastrulation. The analysis of ppt mutant embryos reveals that Ppt/Wnt5 regulates cell elongation and convergent extension movements in posterior regions of the gastrula, while its function in more anterior regions is largely redundant to that of Slb/Wnt11. Frizzled-2 functions downstream of ppt/wnt5, indicating that it might act as a receptor for Ppt/Wnt5 in this process. The characterisation of the role of Ppt/Wnt5 provides insight into the functional diversity of Wnt genes in regulating vertebrate gastrulation movements.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Lineage
  • Gastrula / metabolism
  • Gastrula / physiology*
  • Gene Expression Regulation, Developmental*
  • Glycoproteins / genetics
  • Glycoproteins / physiology
  • In Situ Hybridization
  • Microscopy, Confocal
  • Mutation
  • Oligonucleotides, Antisense / pharmacology
  • Phenotype
  • Proto-Oncogene Proteins / physiology*
  • Receptors, Neurotransmitter / metabolism
  • Signal Transduction
  • Time Factors
  • Transcription Factors / genetics
  • Transcription Factors / physiology*
  • Wnt Proteins
  • Wnt-5a Protein
  • Zebrafish
  • Zebrafish Proteins / physiology*

Substances

  • Glycoproteins
  • Oligonucleotides, Antisense
  • Proto-Oncogene Proteins
  • Receptors, Neurotransmitter
  • Transcription Factors
  • Wnt Proteins
  • Wnt-5a Protein
  • Wnt11 protein, zebrafish
  • Wnt5a protein, zebrafish
  • Zebrafish Proteins