Characterization of mouse muc6 and evidence of conservation of the gel-forming mucin gene cluster between human and mouse

Genomics. 2003 Apr;81(4):433-6. doi: 10.1016/s0888-7543(03)00036-3.


Using degenerate primers designed from conserved cysteine-rich domains of gel-forming mucins, we cloned two new mouse mucin cDNAs. Blast searching showed that they belong to the same new gene assigned to chromosome 7 band F5. This gene is clustered with the three secreted large gel-forming mucins Muc2, Muc5ac, and Muc5b in a region that exhibits synteny with human chromosome 11p15. Computer analysis and sequence alignments with mucin genes predict that the new gene is composed of 33 exons and spans 30 kb from the initiation ATG codon to the Stop codon. Sequence similarities, domain organization of the deduced peptide, and expression analysis allow us to conclude that this newly cloned mouse gene is Muc6, i.e., the mouse ortholog of human MUC6. Like those of their human homologs, the genomic order and arrangement of the four mucins within the cluster of mucin genes are conserved.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Blotting, Southern
  • Chromosome Mapping*
  • DNA Primers
  • Gene Expression Profiling*
  • Humans
  • Mice
  • Molecular Sequence Data
  • Mucin-6
  • Mucins / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction


  • DNA Primers
  • MUC6 protein, human
  • Muc6 protein, mouse
  • Mucin-6
  • Mucins

Associated data

  • GENBANK/AF533526
  • GENBANK/AY184385
  • GENBANK/AY184386
  • GENBANK/AY184387
  • GENBANK/AY184388