C. elegans ORFeome version 1.1: experimental verification of the genome annotation and resource for proteome-scale protein expression

Nat Genet. 2003 May;34(1):35-41. doi: 10.1038/ng1140.


To verify the genome annotation and to create a resource to functionally characterize the proteome, we attempted to Gateway-clone all predicted protein-encoding open reading frames (ORFs), or the 'ORFeome,' of Caenorhabditis elegans. We successfully cloned approximately 12,000 ORFs (ORFeome 1.1), of which roughly 4,000 correspond to genes that are untouched by any cDNA or expressed-sequence tag (EST). More than 50% of predicted genes needed corrections in their intron-exon structures. Notably, approximately 11,000 C. elegans proteins can now be expressed under many conditions and characterized using various high-throughput strategies, including large-scale interactome mapping. We suggest that similar ORFeome projects will be valuable for other organisms, including humans.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alternative Splicing
  • Animals
  • Caenorhabditis elegans / genetics*
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • DNA, Helminth / genetics
  • Databases, Genetic
  • Exons
  • Expressed Sequence Tags
  • Gene Expression
  • Genes, Helminth
  • Genome*
  • Genomics
  • Helminth Proteins / genetics
  • Humans
  • Introns
  • Open Reading Frames
  • Proteome
  • Proteomics


  • DNA, Complementary
  • DNA, Helminth
  • Helminth Proteins
  • Proteome