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. 2003 Mar;11(3):149-52.

[Immune Response of Dendritic Cells Acquiring Antigens From Apoptotic Cholangiocarcinoma Cells Induced by Mitomycin]

[Article in Chinese]
Affiliations
  • PMID: 12681060

[Immune Response of Dendritic Cells Acquiring Antigens From Apoptotic Cholangiocarcinoma Cells Induced by Mitomycin]

[Article in Chinese]
Gang Wu et al. Zhonghua Gan Zang Bing Za Zhi. .

Abstract

Objective: To investigate the anti-tumor immune response of dendritic cells (DCs) acquiring antigens from apoptotic cholangiocarcinoma cells and their therapeutic effects on cholangiocarcinoma cells.

Methods: DCs from human peripheral blood monocytes which acquired antigen capturing and processing capacity, characteristics of maturation, were established in vitro using granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4). Then cholangiocarcinoma cells were induced to apoptosis with mitomycin. The three groups included (1) coculture of DCs, apoptotic cancer cells and T cells, (2) coculture of DCs, necrotic cancer cells and T cells, (3) coculture of DCs, cultured cancer cells and T cells. After 7 days, DCs and T cells were riched separately to perform anti-tumor cells test and immune response test.

Results: these cells had typical dendritic cell morphology, expressed high levels of CD1a and B7, acquired antigen from apoptotic cells caused by mitomycin and could stimulate T cells to inhibit, even kill cholangiocarcinoma cells.

Conclusions: The DCs from peripheral blood monocytes induced by GM-CSF and IL-4 can efficiently present antigen derived from apoptotic cells caused by mitomycin, and stimulate T cells activity obviously. It maybe become an effective therapy for tumor.

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