Interaction of Sedlin with chloride intracellular channel proteins

FEBS Lett. 2003 Apr 10;540(1-3):77-80. doi: 10.1016/s0014-5793(03)00228-x.


Sedlin is an evolutionarily conserved protein encoded by the causative gene SEDL for spondyloepiphyseal dysplasia tarda. Nevertheless, how Sedlin mutations cause the disease remains unknown. Here, the intracellular chloride channel protein CLIC1 was shown to associate with Sedlin by yeast two-hybrid screening. Green fluorescence protein-CLIC1 readily co-immunoprecipitated with FLAG-Sedlin. In addition, both proteins colocalized extensively in cytoplasmic vesicular/reticular structures in COS-7 cells, suggesting their interaction at intracellular membranous organelles. Sedlin also associated with CLIC2 in yeast two-hybrid assays. The link between Sedlin and the intracellular chloride channels is the first step to understand their functional interplays.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • COS Cells
  • Carrier Proteins / metabolism*
  • Chloride Channels / metabolism*
  • Membrane Transport Proteins*
  • Protein Binding
  • Transcription Factors


  • Carrier Proteins
  • Chloride Channels
  • Membrane Transport Proteins
  • TRAPPC2 protein, human
  • Transcription Factors